Comparison of comparative genomic hybridization technologies across microarray platforms.
J Biomol Tech
; 20(2): 135-51, 2009 Apr.
Article
en En
| MEDLINE
| ID: mdl-19503625
ABSTRACT
In the 2007 Association of Biomolecular Resource Facilities Microarray Research Group project, we analyzed HL-60 DNA with five platforms Agilent, Affymetrix 500K, Affymetrix U133 Plus 2.0, Illumina, and RPCI 19K BAC arrays. Copy number variation was analyzed using circular binary segmentation (CBS) analysis of log ratio scores from four independently assessed hybridizations of each platform. Data obtained from these platforms were assessed for reproducibility and the ability to detect formerly reported copy number variations in HL-60. In HL-60, all of the tested platforms detected genomic DNA amplification of the 8q24 locus, trisomy 18, and monosomy X; and deletions at loci 5q11.2~q31, 9p21.3~p22, 10p12~p15, 14q22~q31, and 17p12~p13.3. In the HL-60 genome, at least two of the five platforms detected five novel losses and five novel gains. This report provides guidance in the selection of platforms based on this wide-ranging evaluation of available CGH platforms.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Aberraciones Cromosómicas
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Hibridación Genómica Comparativa
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
J Biomol Tech
Asunto de la revista:
BIOTECNOLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos