Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2.
Biomaterials
; 31(20): 5385-96, 2010 Jul.
Article
en En
| MEDLINE
| ID: mdl-20398929
ABSTRACT
Ex vivo expansion of hematopoietic stem cells has been explored in the fields of stem cell biology, gene therapy and clinical transplantation. Recently, we demonstrated the existence of a circulating myogenic progenitor expressing the CD133 antigen. The relative inability of circulating CD133+ stem cells to reproduce themselves ex vivo imposes substantial limitations on their use for clinical applications in muscular dystrophies. Here we report that the use of cluster-assembled nanostructured titanium dioxide (ns-TiO(2)) substrates, in combination with cytokine enriched medium, enables high-level expansion of circulating CD133+ stem cells in vitro. Furthermore, we demonstrate that expanded circulating CD133+ stem cells retain their in vitro capacity to differentiate into myogenic cells. The exploitation of cluster-assembled ns-TiO(2) substrates for the expansion of CD133+ stem cells in vitro could therefore make the clinical application of these stem cells for the treatment of muscle diseases practical.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Madre
/
Titanio
/
Movimiento Celular
/
Células Musculares
/
Nanoestructuras
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biomaterials
Año:
2010
Tipo del documento:
Article
País de afiliación:
Italia