Molecular mechanism of the interactions between white spot syndrome virus anti-apoptosis protein AAP-1 (WSSV449) and shrimp effector caspase.
Dev Comp Immunol
; 34(10): 1068-74, 2010 Oct.
Article
en En
| MEDLINE
| ID: mdl-20546774
ABSTRACT
AAP-1 (WSSV449), an anti-apoptosis protein encoded by white spot syndrome virus (WSSV), blocked apoptosis in insect cells (SF9) induced by Penaeus monodon effector caspase (Pm caspase). Here, to characterize in detail the anti-Pm caspase activity of AAP-1, both proteins were expressed and purified from Escherichia coli and their interactions were assayed in vitro. We found that although AAP-1 could inhibit Pm caspase activity, the inhibition was not as efficient as that of baculovirus anti-apoptosis protein P35. We further confirmed the binding and cleavage of AAP-1 by Pm caspase, and detected three AAP-1 cleavage products. Mutational analysis and protein N-terminal sequencing revealed that whereas both Asp233 and Asp272 residues of AAP-1 are involved in binding and cleavage by Pm caspase, only the Asp272 is involved in Pm caspase inhibition. Asp233, on the other hand, negatively regulates AAP-1's anti-Pm caspase activity. Lastly, AAP-1 homotypically interacts with each other both in vitro and in insect cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pandalidae
/
Virus del Síndrome de la Mancha Blanca 1
/
Infecciones por Virus ADN
/
Proteínas Reguladoras de la Apoptosis
/
Caspasas Efectoras
/
Proteínas Reguladoras y Accesorias Virales
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
2010
Tipo del documento:
Article