Toward next generation plasma profiling via heat-induced epitope retrieval and array-based assays.
Mol Cell Proteomics
; 9(11): 2497-507, 2010 Nov.
Article
en En
| MEDLINE
| ID: mdl-20682762
ABSTRACT
There is a need for high throughput methods for screening patient samples in the quest for potential biomarkers for diagnostics and patient care. Here, we used a combination of undirected target selection, antibody suspension bead arrays, and heat-induced epitope retrieval to allow for protein profiling of human plasma in a novel and systematic manner. Several antibodies were found to reveal altered protein profiles upon epitope retrieval at elevated temperatures with limits of detection improving into lower ng/ml ranges. In a study based on prostate cancer patients, several proteins with differential profiles were discovered and subsequently validated in an independent cohort. For one of the potential biomarkers, the human carnosine dipeptidase 1 protein (CNDP1), the differences were determined to be related to the glycosylation status of the targeted protein. The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Sanguíneas
/
Análisis por Matrices de Proteínas
/
Ensayos Analíticos de Alto Rendimiento
/
Calor
/
Epítopos
Tipo de estudio:
Evaluation_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
Mol Cell Proteomics
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Suecia