Development of improved methods for delivery of Trichuris muris to the laboratory mouse.
Parasitol Res
; 107(5): 1103-13, 2010 Oct.
Article
en En
| MEDLINE
| ID: mdl-20809420
ABSTRACT
Murine immunological responses to experimental infection with Trichuris muris and the effects of the resident microbiota on these responses are of increasing interest. For these studies, accurate dose delivery and improved sterilization of inocula are essential to prevent co-infection with unknown contaminants. We found that washing T. muris eggs with antibiotics may not be sufficient for sterilization of inocula. However, washing eggs in 6.25% hypochlorite/bleach eliminated bacteria and fungi, as determined by culture and PCR, did not harm viable T. muris eggs and reduced the number of non-viable eggs in the inocula. A hatching assay and propidium iodide staining method were developed and found to increase the accuracy for assessing T. muris egg viability prior to infection for rapid dose evaluation. In addition, metal gavage feeding needles increased the accuracy and precision of the dose delivered to the mice compared to flexible rubber tubes. These methods will improve experimental Trichuris studies by decreasing the variability in outcome due to unintended carryover of adherent microorganisms and unrecognized variation in inocula.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tricuriasis
/
Trichuris
/
Modelos Animales de Enfermedad
/
Interacciones Huésped-Parásitos
Límite:
Animals
Idioma:
En
Revista:
Parasitol Res
Asunto de la revista:
PARASITOLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos