[Study of calcium buffer in SH-SY5Y cells].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 17(4): 352-5, 2001 Nov.
Article
en Zh
| MEDLINE
| ID: mdl-21207698
ABSTRACT
AIM:
To study the calcium buffer in SH-SY5Y cells.METHODS:
By using patch clamp technique, measure voltage-gated calcium currents in undifferentiated SH-SY5Y cell line. Then, by using microfluorometric technique, detect the intracellular [Ca2+]i and the dynamic recovery course after high-K+ induced [Ca2+]i elevation.RESULTS:
There are voltage-gated calcium currents in undifferentiated SH-SYSY cell line. When the time intervals between the stimulus are < 150s, the recovery course will be much more deferred because of the saturation of the intracellular calcium buffer; and when the intervals between the stimulus are > 150 s, the buffer will recruit so as to lead to the steadiness state of the recover course.CONCLUSION:
Calcium buffer proteins have an important effect in the course of cellular calcium signal transduction.
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Banco de datos:
MEDLINE
Asunto principal:
Canales de Calcio
/
Calcio
/
Señalización del Calcio
/
Neuroblastoma
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Ying Yong Sheng Li Xue Za Zhi
Asunto de la revista:
FISIOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
China