[Study of calcium buffer in SH-SY5Y cells].

ABSTRACT

AIM: To study the calcium buffer in SH-SY5Y cells. METHODS: By using patch clamp technique, measure voltage-gated calcium currents in undifferentiated SH-SY5Y cell line. Then, by using microfluorometric technique, detect the intracellular [Ca2+]i and the dynamic recovery course after high-K+ induced [Ca2+]i elevation. RESULTS: There are voltage-gated calcium currents in undifferentiated SH-SYSY cell line. When the time intervals between the stimulus are < 150s, the recovery course will be much more deferred because of the saturation of the intracellular calcium buffer; and when the intervals between the stimulus are > 150 s, the buffer will recruit so as to lead to the steadiness state of the recover course. CONCLUSION: Calcium buffer proteins have an important effect in the course of cellular calcium signal transduction.