Binding kinetics of biomolecule interaction at ultralow concentrations based on gold nanoparticle enhancement.
Anal Chem
; 83(9): 3290-6, 2011 May 01.
Article
en En
| MEDLINE
| ID: mdl-21466206
ABSTRACT
Measuring the kinetic constants of protein-protein interactions at ultralow concentrations becomes critical in characterizing biospecific affinity, and exploring the feasibility of clinical diagnosis with respect to detection sensitivity, efficiency and accuracy. In this study, we propose a method that can calculate the binding constants of protein-protein interactions in sandwich assays at ultralow concentrations at the pg/mL level, using a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB). We discuss a two-compartment model to achieve reaction-limited kinetics under the stagnant conditions of the reaction chamber. The association rate constant, dissociation rate constant, and the equilibrium dissociation constant, that is, k(a), k(d), K(D), respectively, of the kinetics of binding between total prostate-specific antigen (t-PSA) and anti-t-PSA at concentrations from 0.1 pg/mL to 1 ng/mL, were measured either in PBS or in human serum. This is the first time that k(a), k(d), and K(D) have been measured at such a low concentration range in a complex sample such as human serum.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Nanopartículas del Metal
/
Oro
Límite:
Animals
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Humans
Idioma:
En
Revista:
Anal Chem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Taiwán