The styryl dye FM1-43 suppresses odorant responses in a subset of olfactory neurons by blocking cyclic nucleotide-gated (CNG) channels.
J Biol Chem
; 286(32): 28041-8, 2011 Aug 12.
Article
en En
| MEDLINE
| ID: mdl-21646359
ABSTRACT
Many olfactory receptor neurons use a cAMP-dependent transduction mechanism to transduce odorants into depolarizations. This signaling cascade is characterized by a sequence of two currents a cation current through cyclic nucleotide-gated channels followed by a chloride current through calcium-activated chloride channels. To date, it is not possible to interfere with these generator channels under physiological conditions with potent and specific blockers. In this study we identified the styryl dye FM1-43 as a potent blocker of native olfactory cyclic nucleotide-gated channels. Furthermore, we characterized this substance to stain olfactory receptor neurons that are endowed with cAMP-dependent transduction. This allows optical differentiation and pharmacological interference with olfactory receptor neurons at the level of the signal transduction.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Vías Olfatorias
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Compuestos de Piridinio
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AMP Cíclico
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Transmisión Sináptica
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Colorantes Fluorescentes
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Compuestos de Amonio Cuaternario
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Canales Iónicos
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Neuronas
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania