Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 68(Pt 1): 20-3, 2012 Jan 01.
Article
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| MEDLINE
| ID: mdl-22232164
ABSTRACT
In order to clarify the structure and the peptide-presentation characteristics of the equine major histocompatibility complex (MHC) class I molecule, a complex of equine MHC class I molecule (ELA-A1 haplotype, 7-6 allele) with mouse ß(2)-microglobulin and the cytotoxic T lymphocyte (CTL) epitope Env-RW12 (RVEDVTNTAEYW) derived from equine infectious anaemia virus (EIAV) envelope protein (residues 195-206) was refolded and crystallized. The crystal, which belonged to space group P2(1), diffracted to 2.3 Å resolution and had unit-cell parameters a = 82.5, b = 71.4, c = 99.8 Å, ß = 102.9°. The crystal structure contained two molecules in the asymmetric unit. These results should help to determine the first equine MHC class I molecule structure presenting an EIAV CTL epitope.
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1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos T Citotóxicos
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Antígenos de Histocompatibilidad Clase I
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Productos del Gen env
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Virus de la Anemia Infecciosa Equina
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Epítopos de Linfocito T
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Caballos
Límite:
Animals
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Año:
2012
Tipo del documento:
Article