Derivation of iPSCs in stirred suspension bioreactors.
Nat Methods
; 9(5): 465-6, 2012 Apr 08.
Article
en En
| MEDLINE
| ID: mdl-22484846
ABSTRACT
Induced pluripotent stem cells (iPSCs) are typically derived in adherent culture. Here we report fast and efficient derivation of mouse iPSCs in stirred suspension bioreactors, with and without the use of c-Myc. Suspension-reprogrammed cells expressed pluripotency markers, showed multilineage differentiation in vitro and in vivo, and contributed to the germline in chimeric mice. Suspension reprogramming has the potential to accelerate and standardize iPSC research.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
/
Células Madre Pluripotentes Inducidas
Límite:
Animals
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2012
Tipo del documento:
Article
País de afiliación:
Canadá