DOCK8 functions as an adaptor that links TLR-MyD88 signaling to B cell activation.
Nat Immunol
; 13(6): 612-20, 2012 May 13.
Article
en En
| MEDLINE
| ID: mdl-22581261
ABSTRACT
The adaptors DOCK8 and MyD88 have been linked to serological memory. Here we report that DOCK8-deficient patients had impaired antibody responses and considerably fewer CD27(+) memory B cells. B cell proliferation and immunoglobulin production driven by Toll-like receptor 9 (TLR9) were considerably lower in DOCK8-deficient B cells, but those driven by the costimulatory molecule CD40 were not. In contrast, TLR9-driven expression of AICDA (which encodes the cytidine deaminase AID), the immunoglobulin receptor CD23 and the costimulatory molecule CD86 and activation of the transcription factor NF-κB, the kinase p38 and the GTPase Rac1 were intact. DOCK8 associated constitutively with MyD88 and the tyrosine kinase Pyk2 in normal B cells. After ligation of TLR9, DOCK8 became tyrosine-phosphorylated by Pyk2, bound the Src-family kinase Lyn and linked TLR9 to a Src-kinase Syk-transcription factor STAT3 cascade essential for TLR9-driven B cell proliferation and differentiation. Thus, DOCK8 functions as an adaptor in a TLR9-MyD88 signaling pathway in B cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos B
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Factores de Intercambio de Guanina Nucleótido
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Receptor Toll-Like 9
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Factor 88 de Diferenciación Mieloide
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Memoria Inmunológica
Límite:
Adolescent
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Animals
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Child
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Child, preschool
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Humans
Idioma:
En
Revista:
Nat Immunol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos