Hepatitis E virus seroprevalence of domestic pigs in Germany determined by a novel in-house and two reference ELISAs.
J Virol Methods
; 190(1-2): 11-6, 2013 Jun.
Article
en En
| MEDLINE
| ID: mdl-23523888
ABSTRACT
Autochthonous hepatitis E virus (HEV) infections by zoonotic transmission of genotype 3 (GT3) have been reported increasingly from industrialized countries. In this paper the development and validation of an IgG ELISA for the detection of HEV-specific antibodies in domestic pigs is described. Comparison of the diagnostic value of Escherichia coli-expressed HEV-GT3 capsid protein (CP) derivatives revealed a carboxy-terminal derivative as most suitable. Validation of the in-house assay using a commercially available IgG ELISA revealed a high diagnostic specificity and sensitivity. The average HEV seroprevalence of domestic pigs from Germany and the federal state Baden-Wuerttemberg determined by the in-house test was 42.7% and 50.3%, respectively. The seroprevalence in different districts of Baden-Wuerttemberg ranged from 34.9% to 60%, but from 0% to 100% between different herds. These data were compared to those achieved by two commercially available ELISA kits and an in-house ratHEV-based ELISA. In conclusion, the CP-based in-house test proved sensitive and specific, indicating that the ORF3-encoded protein might be dispensable for diagnostics. The novel assay also allowed a parallel analysis by a homologous ratHEV-derived antigen. Thus, the novel IgG ELISA represents a useful tool for future standardized seroprevalence studies in domestic pigs from Germany and other regions of Europe.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de los Porcinos
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Medicina Veterinaria
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Virología
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Hepatitis E
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Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Virol Methods
Año:
2013
Tipo del documento:
Article
País de afiliación:
Alemania