The mathematics of a successful deconvolution: a quantitative assessment of mixture-based combinatorial libraries screened against two formylpeptide receptors.
Molecules
; 18(6): 6408-24, 2013 May 30.
Article
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| MEDLINE
| ID: mdl-23722730
ABSTRACT
In the past 20 years, synthetic combinatorial methods have fundamentally advanced the ability to synthesize and screen large numbers of compounds for drug discovery and basic research. Mixture-based libraries and positional scanning deconvolution combine two approaches for the rapid identification of specific scaffolds and active ligands. Here we present a quantitative assessment of the screening of 32 positional scanning libraries in the identification of highly specific and selective ligands for two formylpeptide receptors. We also compare and contrast two mixture-based library approaches using a mathematical model to facilitate the selection of active scaffolds and libraries to be pursued for further evaluation. The flexibility demonstrated in the differently formatted mixture-based libraries allows for their screening in a wide range of assays.
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MEDLINE
Asunto principal:
Biblioteca de Péptidos
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Receptores de Formil Péptido
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Ensayos Analíticos de Alto Rendimiento
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Modelos Teóricos
Idioma:
En
Revista:
Molecules
Asunto de la revista:
BIOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos