Biocatalytically active silCoat-composites entrapping viable Escherichia coli.
Appl Microbiol Biotechnol
; 98(4): 1557-66, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24257838
ABSTRACT
Application of whole cells in industrial processes requires high catalytic activity, manageability, and viability under technical conditions, which can in principle be accomplished by appropriate immobilization. Here, we report the identification of carrier material allowing exceptionally efficient adsorptive binding of Escherichia coli whole cells hosting catalytically active carbonyl reductase from Candida parapsilosis (CPCR2). With the immobilizates, composite formation with both hydrophobic and hydrophilized silicone was achieved, yielding advanced silCoat-material and HYsilCoat-material, respectively. HYsilCoat-whole cells were viable preparations with a cell loading up to 400 mg(E. coli) · g(-1)(carrier) and considerably lower leaching than native immobilizates. SilCoat-whole cells performed particularly well in neat substrate exhibiting distinctly increased catalytic activity.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Biotecnología
/
Escherichia coli
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Alemania