Dietary phytosterol does not accumulate in the arterial wall and prevents atherosclerosis of LDLr-KO mice.
Atherosclerosis
; 231(2): 442-7, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-24267264
ABSTRACT
SCOPE There have been conflicting reports on the usefulness of phytosterols (PS) in preventing atherosclerosis. We evaluated the effects of dietary PS supplementation in LDLr-KO male mice on the plasma and aorta sterol concentrations and on atherosclerotic lesion development. METHODS AND RESULTS:
Mice were fed a high fat diet (40% of energy) supplemented with or without PS (2% w/w, n = 10). Plasma and arterial wall cholesterol and PS concentrations, lesion area, macrophage infiltration, and mRNA expression from LOX-1, CD36, ABCA1 and ABCG1 in peritoneal macrophages were measured. After 16 weeks, the plasma cholesterol concentration in PS mice was lower than that in the controls (p = 0.02) and in the arterial wall (p = 0.03). Plasma PS concentrations were higher in PS-fed animals than in controls (p < 0.0001); however, the arterial wall PS concentration did not differ between groups. The atherosclerotic lesion area in the PS group (n = 5) was smaller than that in controls (p = 0.0062) and the macrophage area (p = 0.0007). PS correlates negatively with arterial lipid content and macrophage (r = -0.76; p < 0.05). PS supplementation induced lower ABCG1 mRNA expression (p < 0.05).CONCLUSIONS:
Despite inducing an increase in PS plasma concentration, PS supplementation is not associated with its accumulation in the arterial wall and prevents atherosclerotic lesion development.Palabras clave
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1
Banco de datos:
MEDLINE
Asunto principal:
Fitosteroles
/
Arterias
/
Receptores de LDL
/
Aterosclerosis
Límite:
Animals
Idioma:
En
Revista:
Atherosclerosis
Año:
2013
Tipo del documento:
Article
País de afiliación:
Brasil