Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope.
Opt Express
; 21(25): 31604-14, 2013 Dec 16.
Article
en En
| MEDLINE
| ID: mdl-24514733
ABSTRACT
In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) of the studied microscope varies from 59 µm × 93 µm up to 178 µm × 280 µm, while the corresponding lateral resolution varies from 0.6 µm to 2 µm for two-photon fluorescence (2PF) signals. With a 34/s video frame rate, in vivo dynamic observation of zebrafish heartbeat through 2PF of the excited green fluorescence protein (GFP) is demonstrated.
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MEDLINE
Asunto principal:
Aumento de la Imagen
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Discos Compactos
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Microscopía de Fluorescencia por Excitación Multifotónica
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En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2013
Tipo del documento:
Article