Metabolic status of pluripotent cells and exploitation for growth in stirred suspension bioreactors.

ABSTRACT

Pluripotent stem cells are of great interest in the field of regenerative medicine. Recent studies have shown that they maintain a glycolytic metabolic status while pluripotent and wholesale changes to mitochondrial and metabolic profile occur during differentiation. This article reviews the process and how this may be exploited in a stirred suspension bioreactor for rapid growth while maintaining pluripotency.