Genetic diversity and population structure of wild Dipsacus asperoides in China as indicated by ISSR markers.
Genet Mol Res
; 13(3): 6340-9, 2014 Feb 14.
Article
en En
| MEDLINE
| ID: mdl-24615115
ABSTRACT
In order to evaluate the genetic diversity and genetic structure of wild Dipsacus asperoides, we surveyed genetic polymorphisms in 288 individuals from 12 populations using ISSR. A total of 240 bands were amplified, among which 190 were polymorphic loci. At the species level, genetic diversity was found to be abundant PPB = 79.17%, NE = 1.2152, H = 0.1361, and Hsp = 0.2213. At the population level, genetic diversity was lower PPB = 30.76 %, NE = 1.0786, H = 0.0897, and Hpop = 0.1375. The calculated Nei genetic differentiation coefficient was 0.3406, which is consistent with the calculated Shannon population genetic differentiation coefficient of 0.3787. This is commonly taken to indicate that most of the genetic variation existed within the populations. Gene flow was calculated as Nm = 0.9679, suggesting that gene exchange only occurred at a low level. Based on the Nei genetic distance, the 12 populations were divided into 4 categories. Our results suggest that D. asperoides has abundant genetic diversity and provides a foundation for the protection and improvement of germplasm resources.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polimorfismo Genético
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Repeticiones de Microsatélite
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Dipsacaceae
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Sitios Genéticos
País/Región como asunto:
Asia
Idioma:
En
Revista:
Genet Mol Res
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
China