NMDA-induced accumulation of Shank at the postsynaptic density is mediated by CaMKII.
Biochem Biophys Res Commun
; 450(1): 808-11, 2014 Jul 18.
Article
en En
| MEDLINE
| ID: mdl-24952157
ABSTRACT
Shank is a specialized scaffold protein present in high abundance at the postsynaptic density (PSD). Using pre-embedding immunogold electron microscopy on cultured hippocampal neurons, we had previously demonstrated further accumulation of Shank at the PSD under excitatory conditions. Here, using the same experimental protocol, we demonstrate that a cell permeable CaMKII inhibitor, tatCN21, blocks NMDA-induced accumulation of Shank at the PSD. Furthermore we show that NMDA application changes the distribution pattern of Shank at the PSD, promoting a 7-10 nm shift in the median distance of Shank labels away from the postsynaptic membrane. Inhibition of CaMKII with tatCN21 also blocks this shift in the distribution of Shank. Altogether these results imply that upon activation of NMDA receptors, CaMKII mediates accumulation of Shank, preferentially at the distal regions of the PSD complex extending toward the cytoplasm.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
N-Metilaspartato
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Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina
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Densidad Postsináptica
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Hipocampo
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Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos