A fully validated method for the determination of lacosamide in human plasma using gas chromatography with mass spectrometry: application for therapeutic drug monitoring.
J Sep Sci
; 38(2): 260-6, 2015 Jan.
Article
en En
| MEDLINE
| ID: mdl-25395063
ABSTRACT
A simple gas chromatographic method with mass spectrometry detection was developed and validated for the determination of lacosamide in human plasma. Lacosamide and the internal standard, levetiracetam-d6, were extracted from 200 µL plasma, by a solid-phase extraction through HF Bond Elut C18 columns, and derivatized using N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide with 1% tert-butyldimethylsilylchloride in acetonitrile. The limit of quantification was found to be 0.20 µg/mL and the assay was linear up to 20.0 µg/mL with correlation coefficient ≥0.994. The intra- and interday precision values were <4.1% in terms of relative standard deviation (%) and the values of intra- and interday accuracy were found to be within -7.2 and 5.3% in terms of relative error (%). Absolute recovery of the method for lacosamide was determined at three concentration levels and ranged from 92.5 to 97.6%. The developed method uses small volumes of plasma and proved to be simple, rapid, and sensitive for the determination of lacosamide in plasma. This method can be used in routine every day analysis of plasma samples obtained from patients who follow respective antiepileptic treatment and for the investigation of clinical and forensic cases where lacosamide is involved.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Monitoreo de Drogas
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Epilepsia
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Acetamidas
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Cromatografía de Gases y Espectrometría de Masas
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Anticonvulsivantes
Límite:
Humans
Idioma:
En
Revista:
J Sep Sci
Año:
2015
Tipo del documento:
Article
País de afiliación:
Grecia