Fabrication of perforated isoporous membranes via a transfer-free strategy: enabling high-resolution separation of cells.
ACS Appl Mater Interfaces
; 6(24): 22400-7, 2014 Dec 24.
Article
en En
| MEDLINE
| ID: mdl-25421306
ABSTRACT
Thin perforated membranes with ordered pores are ideal barriers for high-resolution and high-efficiency selective transport and separation of biological species. However, for self-assembled thin membranes with a thickness less than several micrometers, an additional step of transferring the membranes onto porous supports is generally required. In this article, we present a facile transfer-free strategy for fabrication of robust perforated composite membranes via the breath figure process, and for the first time, demonstrate the application of the membranes in high-resolution cell separation of yeasts and lactobacilli without external pressure, achieving almost 100% rejection of yeasts and more than 70% recovery of lactobacilli with excellent viability. The avoidance of the transfer step simplifies the fabrication procedure of composite membranes and greatly improves the membrane homogeneity. Moreover, the introduction of an elastic triblock copolymer increases the interfacial strength between the membrane and the support, and allows the preservation of composite membranes in a dry state. Such perforated ordered membranes can also be applied in other size-based separation systems, enabling new opportunities in bioseparation and biosensors.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Poliestirenos
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Levaduras
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Separación Celular
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Streptococcus thermophilus
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Membranas Artificiales
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Metacrilatos
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Nylons
Idioma:
En
Revista:
ACS Appl Mater Interfaces
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2014
Tipo del documento:
Article