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A comprehensive map of genome-wide gene regulation in Mycobacterium tuberculosis.
Turkarslan, Serdar; Peterson, Eliza J R; Rustad, Tige R; Minch, Kyle J; Reiss, David J; Morrison, Robert; Ma, Shuyi; Price, Nathan D; Sherman, David R; Baliga, Nitin S.
Afiliación
  • Turkarslan S; Institute for Systems Biology , Seattle, Washington 98109, USA.
  • Peterson EJ; Institute for Systems Biology , Seattle, Washington 98109, USA.
  • Rustad TR; Seattle Biomedical Research Institute , Seattle, Washington 98109, USA ; Interdisciplinary Program of Pathobiology, Department of Global Health, University of Washington , Seattle, Washington 98195, USA.
  • Minch KJ; Seattle Biomedical Research Institute , Seattle, Washington 98109, USA.
  • Reiss DJ; Institute for Systems Biology , Seattle, Washington 98109, USA.
  • Morrison R; Seattle Biomedical Research Institute , Seattle, Washington 98109, USA.
  • Ma S; Institute for Systems Biology , Seattle, Washington 98109, USA ; Seattle Biomedical Research Institute , Seattle, Washington 98109, USA ; Department of Chemical and Biomolecular Engineering, University of Illinois , Urbana, Illinois 61801, USA.
  • Price ND; Institute for Systems Biology , Seattle, Washington 98109, USA.
  • Sherman DR; Seattle Biomedical Research Institute , Seattle, Washington 98109, USA ; Interdisciplinary Program of Pathobiology, Department of Global Health, University of Washington , Seattle, Washington 98195, USA ; Molecular and Cellular Biology Program, University of Washington , Seattle, Washington 98195, U
  • Baliga NS; Institute for Systems Biology , Seattle, Washington 98109, USA ; Molecular and Cellular Biology Program, University of Washington , Seattle, Washington 98195, USA ; Departments of Biology and Microbiology, University of Washington , Seattle, Washington 98195, USA ; Lawrence Berkeley National Lab , B
Sci Data ; 2: 150010, 2015.
Article en En | MEDLINE | ID: mdl-25977815
ABSTRACT
Mycobacterium tuberculosis (MTB) is a pathogenic bacterium responsible for 12 million active cases of tuberculosis (TB) worldwide. The complexity and critical regulatory components of MTB pathogenicity are still poorly understood despite extensive research efforts. In this study, we constructed the first systems-scale map of transcription factor (TF) binding sites and their regulatory target proteins in MTB. We constructed FLAG-tagged overexpression constructs for 206 TFs in MTB, used ChIP-seq to identify genome-wide binding events and surveyed global transcriptomic changes for each overexpressed TF. Here we present data for the most comprehensive map of MTB gene regulation to date. We also define elaborate quality control measures, extensive filtering steps, and the gene-level overlap between ChIP-seq and microarray datasets. Further, we describe the use of TF overexpression datasets to validate a global gene regulatory network model of MTB and describe an online source to explore the datasets.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Regulación Bacteriana de la Expresión Génica / Genoma Bacteriano / Mycobacterium tuberculosis Tipo de estudio: Prognostic_studies Idioma: En Revista: Sci Data Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Regulación Bacteriana de la Expresión Génica / Genoma Bacteriano / Mycobacterium tuberculosis Tipo de estudio: Prognostic_studies Idioma: En Revista: Sci Data Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos