[Affinity Detection and Quantification of Receptors of TRAIL Based on Their Thermostability].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 46(3): 449-52, 2015 May.
Article
en Zh
| MEDLINE
| ID: mdl-26121872
ABSTRACT
OBJECTIVE:
To develop a way for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors quantification in cancer via its' thermostability.METHODS:
Endougenous alkaline phosphatase (AP) activity and denaturation temperature of pancreatic cancer cell lines AsPC-1 and Capan-2 were detected. Boiling treated recombinant protein death receptor 5 (DR5), named DR5 AP, as well as pancreatic cancer cells lines AsPC-1 and Capan-2 were incubated with AP-tagged TRAIL (AP-TRAIL), and then reacted with Reagent A and Reagent S, the substrate of AP, to quantitive and in site detection of the receptor.RESULTS:
The endougenous AP activity of pancreatic cancer cells lines AsPC-1 and Capan-2 could not be totally inactivated by incubated at 65 °C, thus inhibited the detection of TRAIL receptor, but the activity was dramatically decreased after treated with boiling water, whereas the DR5-AP was thermal stable. The surface receptor of AsPC-1 and Capan-2 could be recognized and bound by AP-TRAIL after treated at 100 °C, the readings were 2. 210±0. 393 and 2. 027±0. 019.CONCLUSION:
The TRAIL receptors are thermostable and this may provide a better diagnosis and prognosis of cancer as well as personalize cancer therapy.
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Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Pancreáticas
/
Receptores del Ligando Inductor de Apoptosis Relacionado con TNF
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2015
Tipo del documento:
Article