Integrin LFA-1 regulates cell adhesion via transient clutch formation.
Biochem Biophys Res Commun
; 464(2): 459-66, 2015 Aug 21.
Article
en En
| MEDLINE
| ID: mdl-26143530
ABSTRACT
Integrin LFA-1 regulates immune cell adhesion and trafficking by binding to ICAM-1 upon chemokine stimulation. Integrin-mediated clutch formation between extracellular ICAM-1 and the intracellular actin cytoskeleton is important for cell adhesion. We applied single-molecule tracking analysis to LFA-1 and ICAM-1 in living cells to examine the ligand-binding kinetics and mobility of the molecular clutch under chemokine-induced physiological adhesion and Mn(2+)-induced tight adhesion. Our results show a transient LFA-1-mediated clutch formation that lasts a few seconds and leads to a transient lower-mobility is sufficient to promote cell adhesion. Stable clutch formation was observed for Mn(2+)-induced high affinity LFA-1, but was not required for physiological adhesion. We propose that fast cycling of the clutch formation by intermediate-affinity integrin enables dynamic cell adhesion and migration.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Adhesión Celular
/
Antígeno-1 Asociado a Función de Linfocito
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2015
Tipo del documento:
Article
País de afiliación:
Japón