Study of intact virus-like particles of human papillomavirus by capillary electrophoresis.
Electrophoresis
; 37(4): 579-86, 2016 Feb.
Article
en En
| MEDLINE
| ID: mdl-26634429
ABSTRACT
Virus-like particles of human papillomavirus (HPV-VLP), resulting from the self-assembly of the capsid proteins (L1 or L1 and L2), have been widely used to study HPV as they are similar to the native virion. Moreover, two prophylactic vaccines, Gardasil(®) and Cervarix(®), are based on HPV-VLP L1. Analytical techniques currently used to characterize HPV-VLP, such as SDS-PAGE, Western blot, ELISA, are time-consuming and semiquantitative. In this study, CE was evaluated for the analysis of intact HPV16-VLP. The usefulness of capillary inner wall coating as well as various BGEs, pH, and detergent additives were investigated. Reproducible HPV-VLP analysis in CE was achieved using poly(ethylene oxide)-coated capillary and a BGE containing high salt concentration and low SDS concentration. The developed method enables HPV-VLP detection in less than 10 min (migration times RSD 1.6%). The identity of HPV-VLP peak was confirmed by comparison with a sample obtained from a wild-type baculovirus and with VLP-based vaccine, Gardasil(®) , after adjuvant dissolution. Finally, we applied the developed methodology to VLP-based vaccines, demonstrating that CE could be successfully used for vaccine quality control.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Virión
/
Virología
/
Electroforesis Capilar
/
Papillomavirus Humano 16
Idioma:
En
Revista:
Electrophoresis
Año:
2016
Tipo del documento:
Article
País de afiliación:
Bélgica