A multisample dissolution dynamic nuclear polarization system for serial injections in small animals.
Magn Reson Med
; 77(2): 904-910, 2017 02.
Article
en En
| MEDLINE
| ID: mdl-26900678
ABSTRACT
PURPOSE:
Several in vivo applications of dissolution dynamic nuclear polarization (DNP) require rapid successive injections of hyperpolarized substrates. Here we present the design and performance of a custom-built multisample dissolution DNP setup for small animal research.METHODS:
The DNP setup consists of a commercial wide-bore magnet charged to 3.35 T, a cryostat, a 94-GHz microwave source, and a custom-built skeleton that accommodates four identical sample sticks. Each sample stick features a dissolver locked into the skeleton port and a lifter, which permits moving the sample cup out of the liquid helium bath for dissolution.RESULTS:
The dissolution of the first sample was triggered after 2 hours of polarization buildup during single-shot operation of the cryostat. Thereafter, a time window of 75-90 min was available to dissolve the remaining three polarized samples. The average liquid state polarization over all four sticks was measured as 18.7% ± 2.3% for [1-13C] pyruvate 30 s after dissolution. In vivo applicability of the setup using serial injections of [1-13C] pyruvate to study cardiac metabolism in rats revealed good reproducibility.CONCLUSION:
The proposed four-sample DNP insert provides reproducible liquid state polarization of [1-13C] pyruvate and allows for rapid repeat injections in small animals. Magn Reson Med 77904-910, 2017. © 2016 International Society for Magnetic Resonance in Medicine.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Imagen por Resonancia Magnética
Límite:
Animals
Idioma:
En
Revista:
Magn Reson Med
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2017
Tipo del documento:
Article
País de afiliación:
Suiza