Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse.
Cell Res
; 26(3): 275-87, 2016 Mar.
Article
en En
| MEDLINE
| ID: mdl-26902285
ABSTRACT
In early mammalian embryos, the genome is transcriptionally quiescent until the zygotic genome activation (ZGA) which occurs 2-3 days after fertilization. Despite a long-standing effort, maternal transcription factors regulating this crucial developmental event remain largely elusive. Here, using maternal and paternal mouse models of Yap1 deletion, we show that maternally accumulated yes-associated protein (YAP) in oocyte is essential for ZGA. Maternal Yap1-knockout embryos exhibit a prolonged two-cell stage and develop into the four-cell stage at a much slower pace than the wild-type controls. Transcriptome analyses identify YAP target genes in early blastomeres; two of which, Rpl13 and Rrm2, are required to mediate maternal YAP's effect in conferring developmental competence on preimplantation embryos. Furthermore, the physiological YAP activator, lysophosphatidic acid, can substantially improve early development of wild-type, but not maternal Yap1-knockout embryos in both oviduct and culture. These observations provide insights into the mechanisms of ZGA, and suggest potentials of YAP activators in improving the developmental competence of cultured embryos in assisted human reproduction and animal biotechnology.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Oocitos
/
Fosfoproteínas
/
Cigoto
/
Regulación del Desarrollo de la Expresión Génica
/
Proteínas Adaptadoras Transductoras de Señales
/
Desarrollo Embrionario
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cell Res
Año:
2016
Tipo del documento:
Article
País de afiliación:
China