Assessment of the Contractile Properties of Permeabilized Skeletal Muscle Fibers.
Methods Mol Biol
; 1460: 321-36, 2016.
Article
en En
| MEDLINE
| ID: mdl-27492182
ABSTRACT
Permeabilized individual skeletal muscle fibers offer the opportunity to evaluate contractile behavior in a system that is greatly simplified, yet physiologically relevant. Here we describe the steps required to prepare, permeabilize and preserve small samples of skeletal muscle. We then detail the procedures used to isolate individual fiber segments and attach them to an experimental apparatus for the purpose of controlling activation and measuring force generation. We also describe our technique for estimating the cross-sectional area of fiber segments. The area measurement is necessary for normalizing the absolute force to obtain specific force, a measure of the intrinsic force-generating capability of the contractile system.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fibras Musculares Esqueléticas
/
Contracción Muscular
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos