Islet Insulin Secretion Measurements in the Mouse.
Curr Protoc Mouse Biol
; 6(3): 256-271, 2016 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-27584553
ABSTRACT
This article describes detailed protocols for in vitro measurements of insulin function and secretion in isolated mouse islets for the analysis of glucose homeostasis. We specify a method of enzyme digestion and hand picking to isolate and release the greatest number of high quality islets from the pancreas of the mouse. We describe an effective method for generating dynamic measurements of insulin secretion using a perifusion assay including a detailed protocol for constructing a peristaltic pump and tubing assembly. In addition we describe an alternative and simple technique for measuring insulin secretion using static incubation of isolated islets. © 2016 by John Wiley & Sons, Inc.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fisiología
/
Islotes Pancreáticos
/
Insulina
/
Ratones
Límite:
Animals
Idioma:
En
Revista:
Curr Protoc Mouse Biol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Reino Unido