Detection of trace amounts of target DNA from massive background of nucleic acids by using the LM-PCR-based preamplification method.

Pan, Xiaoming; Wang, Jing; Zhang, Yanfang; Dong, Ping; Li, Chunchuan; Liang, Xingguo

Pan, Xiaoming; Wang, Jing; Zhang, Yanfang; Dong, Ping; Li, Chunchuan; Liang, Xingguo.

Afiliación

Pan X; College of Food Science and Engineering, Ocean University of China, Qingdao, Peoples' Republic of China.
Wang J; College of Food Science and Engineering, Ocean University of China, Qingdao, Peoples' Republic of China.
Zhang Y; College of Food Science and Engineering, Ocean University of China, Qingdao, Peoples' Republic of China.
Dong P; College of Food Science and Engineering, Ocean University of China, Qingdao, Peoples' Republic of China.
Li C; College of Food Science and Engineering, Ocean University of China, Qingdao, Peoples' Republic of China.
Liang X; College of Food Science and Engineering, Ocean University of China, Qingdao, Peoples' Republic of China.

Biotechnol Appl Biochem ; 64(6): 879-887, 2017 Nov.

Article en En | MEDLINE | ID: mdl-27859653

Asunto(s)

ADN Bacteriano/genética; Klebsiella/genética; Reacción en Cadena de la Polimerasa; Salmonella typhimurium/genética; Vibrio cholerae/genética; Vibrio parahaemolyticus/genética; ADN Bacteriano/aislamiento & purificación

Palabras clave

complex sample; high sensitivity; multiplex detection; preamplification; type IIS restriction endonuclease

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Salmonella typhimurium / Vibrio cholerae / Vibrio parahaemolyticus / ADN Bacteriano / Reacción en Cadena de la Polimerasa / Klebsiella Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biotechnol Appl Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2017 Tipo del documento: Article

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