A critical reevaluation of some assay methods for superoxide dismutase activity.
Free Radic Biol Med
; 4(5): 295-303, 1988.
Article
en En
| MEDLINE
| ID: mdl-2834277
ABSTRACT
We have compared the direct method of pulse radiolysis to the indirect methods of cytochrome c and nitroblue tetrazolium for assaying the superoxide dismutase activity of a compound. We have shown that with pulse radiolysis, where high concentrations of O2- are generated, the "turnover" rate constant, kcat, can be determined directly, while with the indirect methods, where relatively low steady state concentrations of O2- are formed, the value of kcat determined by these methods, can be orders of magnitude lower than that determined directly. The main reason for the lower values obtained with the indirect methods is due to the fast reoxidation of the reduced compound by molecular oxygen. Additional problems which arise with the use of indirect methods for determining superoxide dismutase catalytic activity are discussed.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Superóxido Dismutasa
Idioma:
En
Revista:
Free Radic Biol Med
Asunto de la revista:
BIOQUIMICA
/
MEDICINA
Año:
1988
Tipo del documento:
Article
País de afiliación:
Israel