In Vitro Biochemical Assays for O-GlcNAc-Processing Enzymes.
Chembiochem
; 18(15): 1462-1472, 2017 08 04.
Article
en En
| MEDLINE
| ID: mdl-28474822
ABSTRACT
O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) are the only enzymes that regulate the dynamics of protein O-GlcNAcylation. Protein O-GlcNAcylation is an important post-translational modification (PTM) of nuclear and cytoplasmic proteins with O-linked ß-N-acetyl-glucosamine (O-GlcNAc). O-GlcNAc and its enzymes are involved in a wide variety of cellular processes and are linked to the pathological progression of chronic diseases. Considering their emerging biological significance, systematic and rapid methods to determine the activities of OGT and OGA have become essential, and several chemical/biochemical methods for measuring the activities of these enzymes have been developed. This minireview mainly focuses on the various biochemical assay methods developed to date, while also providing a description of the fundamental principles underlying the monitoring of O-GlcNAc enzyme activities.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Beta-N-Acetilhexosaminidasas
/
N-Acetilglucosaminiltransferasas
/
Pruebas de Enzimas
Límite:
Humans
Idioma:
En
Revista:
Chembiochem
Asunto de la revista:
BIOQUIMICA
Año:
2017
Tipo del documento:
Article