Rapid and affordable genome-wide bisulfite DNA sequencing by XmaI-reduced representation bisulfite sequencing.
Epigenomics
; 9(6): 833-847, 2017 06.
Article
en En
| MEDLINE
| ID: mdl-28488887
ABSTRACT
AIM:
To develop a reduced representation bisulfite sequencing (RRBS) approach for rapid and affordable genome-wide DNA methylation analysis.METHODS:
We have selected restriction endonuclease XmaI to produce RRBS library fragments. After digestion and partial fill-in DNA fragments were ligated to barcoded adapters, bisulfite converted, size-selected, and sequenced on the Ion Torrent Personal Genome Machine. XmaI-RRBS results were compared with the previously published RRBS data.RESULTS:
We have developed an XmaI-RRBS method for rapid and affordable genome-wide DNA methylation analysis, with library preparation taking only 4 days and sequencing possible within 4 h. We have also addressed several challenges in order to further improve the RRBS technology. XmaI-RRBS may be performed on degraded DNA samples and is compatible with the bench-top next-generation sequencing machines.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Metilación de ADN
/
Secuenciación de Nucleótidos de Alto Rendimiento
/
Secuenciación Completa del Genoma
Límite:
Humans
Idioma:
En
Revista:
Epigenomics
Año:
2017
Tipo del documento:
Article
País de afiliación:
Rusia