Colonic Oxidative and Mitochondrial Function in Parkinson's Disease and Idiopathic REM Sleep Behavior Disorder.
Parkinsons Dis
; 2017: 9816095, 2017.
Article
en En
| MEDLINE
| ID: mdl-28660090
ABSTRACT
OBJECTIVE:
To determine potential mitochondrial and oxidative alterations in colon biopsies from idiopathic REM sleep behavior disorder (iRBD) and Parkinson's disease (PD) subjects.METHODS:
Colonic biopsies from 7 iRBD subjects, 9 subjects with clinically diagnosed PD, and 9 healthy controls were homogenized in 5% w/v mannitol. Citrate synthase (CS) and complex I (CI) were analyzed spectrophotometrically. Oxidative damage was assessed either by lipid peroxidation, through malondialdehyde and hydroxyalkenal content by spectrophotometry, or through antioxidant enzyme levels of superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (Gpx1), and catalase (CAT) by western blot. The presence of mitochondrial DNA (mtDNA) deletions was assessed by long PCR and electrophoresis.RESULTS:
Nonsignificant trends to CI decrease in both iRBD (45.69 ± 18.15; 23% decrease) and PD patients (37.57 ± 12.41; 37% decrease) were found compared to controls (59.51 ± 12.52, p NS). Lipid peroxidation was maintained among groups (iRBD 27.46 ± 3.04, PD 37.2 ± 3.92, and controls 31.71 ± 3.94; p NS). Antioxidant enzymes SOD2 (iRBD 2.30 ± 0.92, PD 1.48 ± 0.39, and controls 1.09 ± 0.318) and Gpx1 (iRBD 0.29 ± 0.12, PD 0.56 ± 0.33, and controls 0.38 ± 0.16) did not show significant differences between groups. CAT was only detected in 2 controls and 1 iRBD subject. One iRBD patient presented a single mtDNA deletion.
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Banco de datos:
MEDLINE
Idioma:
En
Revista:
Parkinsons Dis
Año:
2017
Tipo del documento:
Article
País de afiliación:
España