Covalent modifications of the amyloid beta peptide by hydroxynonenal: Effects on metal ion binding by monomers and insights into the fibril topology.
J Inorg Biochem
; 174: 130-136, 2017 09.
Article
en En
| MEDLINE
| ID: mdl-28668508
Amyloid ß peptides (Aß) and metal ions are associated with oxidative stress in Alzheimer's disease (AD). Oxidative stress, acting on ω-6 polyunsaturated fatty acyl chains, produces diverse products, including 4-hydroxy-2-nonenal (HNE), which can covalently modify the Aß that helped to produce it. To examine possible feedback mechanisms involving Aß, metal ions and HNE production, the effects of HNE modification and fibril formation on metal ion binding was investigated. Results indicate that copper(II) generally inhibits the modification of His side chains in Aß by HNE, but that once modified, copper(II) still binds to Aß with high affinity. Fibril formation protects only one of the three His residues in Aß from HNE modification, and this protection is consistent with proposed models of fibril structure. These results provide insight into a network of biochemical reactions that may be operating as a consequence of oxidative stress in AD, or as part of the pathogenic process.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
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Péptidos beta-Amiloides
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Aldehídos
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Metales
Límite:
Humans
Idioma:
En
Revista:
J Inorg Biochem
Año:
2017
Tipo del documento:
Article