Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation.
Methods Mol Biol
; 1660: 343-350, 2017.
Article
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| MEDLINE
| ID: mdl-28828669
ABSTRACT
This chapter describes a method for isolating human salivary extracellular vesicles (EVs) using density gradient ultracentrifugation. Standard protocols established for isolation of EVs from blood or a conditioned medium of cultured cells do not work for whole saliva, due to its viscosity. Therefore, procedures including a pretreatment step and utilizing iodixanol as a gradient material enable EVs to be concentrated to a 1.1 g/ml density. This protocol is compatible with both swing and angle rotors. By employing an angle rotor, which enables high g-force, the centrifugation time was reduced to 4 h from the 17 h required when using a swing rotor.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Saliva
/
Ultracentrifugación
/
Centrifugación por Gradiente de Densidad
/
Vesículas Extracelulares
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2017
Tipo del documento:
Article
País de afiliación:
Japón