Transcriptional accuracy modeling suggests two-step proofreading by RNA polymerase.
Nucleic Acids Res
; 45(20): 11582-11593, 2017 Nov 16.
Article
en En
| MEDLINE
| ID: mdl-29036494
ABSTRACT
We suggest a novel two-step proofreading mechanism with two sequential rounds of proofreading selection in mRNA transcription. It is based on the previous experimental observations that the proofreading RNA polymerase cleaves off transcript fragments of at least 2 nt and that transcript elongation after a nucleotide misincorporation is anomalously slow. Taking these results into account, we extend the description of the accuracy of template guided nucleotide selection beyond previous models of RNA polymerase-dependent DNA transcription. The model derives the accuracy of initial and proofreading base selection from experimentally estimated nearest-neighbor parameters. It is also used to estimate the small accuracy enhancement of polymerase revisiting of previous positions following transcript cleavage.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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ARN Polimerasa II
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ARN Polimerasas Dirigidas por ADN
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Escherichia coli
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2017
Tipo del documento:
Article
País de afiliación:
Suecia