Two-event Transfusion-related Acute Lung Injury Mouse Model.
Bio Protoc
; 5(12)2015 Jun 20.
Article
en En
| MEDLINE
| ID: mdl-29104887
ABSTRACT
Transfusion-related acute lung injury (TRALI) is defined as acute lung injury that occurs within 6 hours of a blood product transfusion. TRALI continues to be a leading cause of transfusion-related mortality and we have developed a mouse model of TRALI to better understand the mechanisms by which injury occurs and to test therapeutic approaches. Our model is a two-event model based on immune priming and the challenge of BALB/c wild-type mice with cognate MHC Class I monoclonal antibody (MHC I mAb). Immune priming with LPS mimics the primed state of recipients (first event) that is important for the development of TRALI. Donor HLA antibodies are frequently implicated in TRALI reactions, and cognate MHC Class I antibody (second event) produces acute lung injury in primed animals. Here, we describe a detailed protocol with high reproducibility within animals.
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Banco de datos:
MEDLINE
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Bio Protoc
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos