Unique Substrate Specificity of SplE Serine Protease from Staphylococcus aureus.

Stach, Natalia; Kalinska, Magdalena; Zdzalik, Michal; Kitel, Radoslaw; Karim, Abdulkarim; Serwin, Karol; Rut, Wioletta; Larsen, Katrine; Jabaiah, Abeer; Firlej, Magdalena; Wladyka, Benedykt; Daugherty, Patrick; Stennicke, Henning; Drag, Marcin; Potempa, Jan; Dubin, Grzegorz

Stach, Natalia; Kalinska, Magdalena; Zdzalik, Michal; Kitel, Radoslaw; Karim, Abdulkarim; Serwin, Karol; Rut, Wioletta; Larsen, Katrine; Jabaiah, Abeer; Firlej, Magdalena; Wladyka, Benedykt; Daugherty, Patrick; Stennicke, Henning; Drag, Marcin; Potempa, Jan; Dubin, Grzegorz.

Afiliación

Stach N; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow 30-387, Poland.
Kalinska M; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow 30-387, Poland.
Zdzalik M; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland.
Kitel R; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow 30-387, Poland.
Karim A; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland; Department of Biology, College of Science, Salahaddin University, Erbil, 44002 Kurdistan, Iraq.
Serwin K; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland.
Rut W; Division of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology, Wroclaw, 50-370, Poland.
Larsen K; Protein Engineering, Novo Nordisk A/S, Maaloev 2880, Denmark.
Jabaiah A; Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA.
Firlej M; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow 30-387, Poland.
Wladyka B; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow 30-387, Poland.
Daugherty P; Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA.
Stennicke H; Protein Engineering, Novo Nordisk A/S, Maaloev 2880, Denmark.
Drag M; Division of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology, Wroclaw, 50-370, Poland.
Potempa J; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland; Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, KY 40202, USA.
Dubin G; Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow 30-387, Poland; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow 30-387, Poland. Electronic address: grzegorz.dubin@uj.edu.pl.

Structure ; 26(4): 572-579.e4, 2018 04 03.

Article en En | MEDLINE | ID: mdl-29526434

Asunto(s)

Proteínas Bacterianas/química; Péptidos/química; Serina Proteasas/química; Staphylococcus aureus/química; Factores de Virulencia/química; Secuencia de Aminoácidos; Proteínas Bacterianas/genética; Proteínas Bacterianas/metabolismo; Sitios de Unión; Clonación Molecular; Cristalografía por Rayos X; Escherichia coli/genética; Escherichia coli/metabolismo; Expresión Génica; Vectores Genéticos/química; Vectores Genéticos/metabolismo; Cinética; Modelos Moleculares; Mutación; Biblioteca de Péptidos; Péptidos/metabolismo; Unión Proteica; Conformación Proteica en Hélice alfa; Conformación Proteica en Lámina beta; Dominios y Motivos de Interacción de Proteínas; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Alineación de Secuencia; Serina Proteasas/genética; Serina Proteasas/metabolismo; Staphylococcus aureus/enzimología; Staphylococcus aureus/patogenicidad; Especificidad por Sustrato; Factores de Virulencia/genética; Factores de Virulencia/metabolismo

Palabras clave

SplE; Staphylococcus aureus; proteases; proteinase; structure; substrate specificity

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Péptidos / Staphylococcus aureus / Proteínas Bacterianas / Factores de Virulencia / Serina Proteasas Tipo de estudio: Prognostic_studies Idioma: En Revista: Structure Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA / BIOTECNOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Polonia

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