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Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses.
Cao, Weiping; Mishina, Margarita; Amoah, Samuel; Mboko, Wadzanai P; Bohannon, Caitlin; McCoy, James; Mittal, Suresh K; Gangappa, Shivaprakash; Sambhara, Suryaprakash.
Afiliación
  • Cao W; a Immunology and Pathogenesis Branch , National Center for Immunization and Respiratory Diseases , Atlanta , GA , USA.
  • Mishina M; b Influenza Division , Centers for Disease Control and Prevention , Atlanta , GA , USA.
  • Amoah S; a Immunology and Pathogenesis Branch , National Center for Immunization and Respiratory Diseases , Atlanta , GA , USA.
  • Mboko WP; b Influenza Division , Centers for Disease Control and Prevention , Atlanta , GA , USA.
  • Bohannon C; c Battelle Memorial Institute , Atlanta , GA , USA.
  • McCoy J; a Immunology and Pathogenesis Branch , National Center for Immunization and Respiratory Diseases , Atlanta , GA , USA.
  • Mittal SK; b Influenza Division , Centers for Disease Control and Prevention , Atlanta , GA , USA.
  • Gangappa S; c Battelle Memorial Institute , Atlanta , GA , USA.
  • Sambhara S; d Department of Comparative Pathobiology , Purdue University , West Lafayette , IN , USA.
Drug Deliv ; 25(1): 773-779, 2018 Nov.
Article en En | MEDLINE | ID: mdl-29542358
Avian influenza virus infection is a serious public health threat and preventive vaccination is the most cost-effective public health intervention strategy. Unfortunately, currently available unadjuvanted avian influenza vaccines are poorly immunogenic and alternative vaccine formulations and delivery strategies are in urgent need to reduce the high risk of avian influenza pandemics. Cationic polymers have been widely used as vectors for gene delivery in vitro and in vivo. In this study, we formulated H5N1 influenza vaccines with GenJet™ or in vivo-jetPEI®, and showed that these formulations significantly enhanced the immunogenicity of H5N1 vaccines and conferred protective immunity in a mouse model. Detailed analyses of adaptive immune responses revealed that both formulations induced mixed TH1/TH2 antigen-specific CD4 T-cell responses, antigen-specific cytotoxic CD8 T-cell and memory B-cell responses. Our findings suggest that cationic polymers merit future development as potential adjuvants for mucosal delivery of poorly immunogenic vaccines.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Vacunas Sintéticas / Sistemas de Liberación de Medicamentos / Subtipo H5N1 del Virus de la Influenza A / Inmunidad Adaptativa / Gripe Aviar / Inmunidad Celular Tipo de estudio: Prognostic_studies Idioma: En Revista: Drug Deliv Asunto de la revista: FARMACOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Vacunas Sintéticas / Sistemas de Liberación de Medicamentos / Subtipo H5N1 del Virus de la Influenza A / Inmunidad Adaptativa / Gripe Aviar / Inmunidad Celular Tipo de estudio: Prognostic_studies Idioma: En Revista: Drug Deliv Asunto de la revista: FARMACOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos