Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases.

Watanabe, Yuko; Furukawa, Eri; Tatsukawa, Hideki; Hashimoto, Hisashi; Kamei, Yasuhiro; Taniguchi, Yoshihito; Hitomi, Kiyotaka

Watanabe, Yuko; Furukawa, Eri; Tatsukawa, Hideki; Hashimoto, Hisashi; Kamei, Yasuhiro; Taniguchi, Yoshihito; Hitomi, Kiyotaka.

Afiliación

Watanabe Y; a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
Furukawa E; a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
Tatsukawa H; a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
Hashimoto H; b Bioscience Biotechnology Center Nagoya University , Nagoya , Japan.
Kamei Y; c National Institute for Basic Biology , Okazaki , Japan.
Taniguchi Y; d Department of Preventive Medicine and Public Health, School of Medicine , Kyorin University , Tokyo , Japan.
Hitomi K; a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.

Biosci Biotechnol Biochem ; 82(7): 1165-1168, 2018 Jul.

Article en En | MEDLINE | ID: mdl-29570037

ABSTRACT

ABSTRACT

Transglutaminase (TG) is an essential enzyme to catalyze cross-linking reactions of epidermal proteins. Recently, we biochemically characterized human skin TG orthologues for medaka (Oryzias latipes), a model fish. By genome editing, gene-modified fishes for the two orthologues were obtained, both of which lack the ordinal enzymes. These fish appeared to exhibit higher susceptibility to osmolality at the period of larvae.

Asunto(s)

Proteínas de Peces/genética; Mutación; Oryzias/fisiología; Concentración Osmolar; Piel/enzimología; Transglutaminasas/genética; Secuencia de Aminoácidos; Animales; Animales Modificados Genéticamente; Femenino; Edición Génica; Humanos; Larva/fisiología; Masculino; Oryzias/genética; Oryzias/crecimiento & desarrollo; Transglutaminasas/química

Palabras clave

KO: knockout; TG: transglutaminase; Tilling: Targeting-induced local lesions in genome method; Transglutaminase; WT: wild-type; medaka; skin

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Concentración Osmolar / Piel / Oryzias / Transglutaminasas / Proteínas de Peces / Mutación Límite: Animals / Female / Humans / Male Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Japón

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