Cross-Linked Aptamer-Lipid Micelles for Excellent Stability and Specificity in Target-Cell Recognition.
Angew Chem Int Ed Engl
; 57(36): 11589-11593, 2018 09 03.
Article
en En
| MEDLINE
| ID: mdl-30079455
ABSTRACT
The specific binding ability of DNA-lipid micelles (DLMs) can be increased by the introduction of an aptamer. However, supramolecular micellar structures based on self-assemblies of amphiphilic DLMs are expected to demonstrate low stability when interacting with cell membranes under certain conditions, which could lead to a reduction in selectivity for targeting cancer cells. We herein report a straightforward cross-linking strategy that relies on a methacrylamide branch to link aptamer and lipid segments. By an efficient photoinduced polymerization process, covalently linked aptamer-lipid units help stabilize the micelle structure and enhance aptamer probe stability, further improving the targeting ability of the resulting nanoassembly. Besides the development of a facile cross-linking method, this study clarifies the relationship between aptamer-lipid concentration and the corresponding binding ability.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Acrilamidas
/
Portadores de Fármacos
/
Reactivos de Enlaces Cruzados
/
Aptámeros de Nucleótidos
/
Lípidos
/
Micelas
Límite:
Humans
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos