In vitro drusen model - three-dimensional spheroid culture of retinal pigment epithelial cells.
J Cell Sci
; 132(4)2018 08 28.
Article
en En
| MEDLINE
| ID: mdl-30082277
ABSTRACT
Age-related macular degeneration (AMD) is a leading cause of blindness in people over 50â
years of age in many developed countries. Drusen are yellowish extracellular deposits beneath retinal pigment epithelium (RPE) found in aging eyes and considered as a biomarker of AMD. However, the biogenesis of drusen has not been elucidated. We reported previously that multicellular spheroids of human RPE cells constructed a well-differentiated monolayer of RPE with a Bruch's membrane. We determined that RPE spheroids exhibited drusen formation between the RPE and Bruch's membrane with expression of many drusen-associated proteins, such as amyloid ß and complement components, the expression of which was altered by a challenge with oxidative stress. Artificial lipofuscin-loaded RPE spheroids yielded drusen more frequently. In the current study, we showed that drusen originates from the RPE. This culture system is an attractive tool for use as an in vitro drusen model, which might help elucidate the biogenesis of drusen and the pathogenesis of related diseases, such as AMD.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Retina
/
Pigmentos Retinianos
/
Lámina Basal de la Coroides
/
Epitelio Pigmentado de la Retina
Límite:
Humans
Idioma:
En
Revista:
J Cell Sci
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón