[UPLC Fingerprint of Gentiana officinalis Root].

ABSTRACT

Objective: To establish UPLC fingerprints of Gentiana officinalis root and Gentiana macrophylla root,and to evaluate the similarity of the fingerprints,in order to provide theoretical basis for the quality control of Gentiana officinalis root. Methods: The separation was performed on an ACQUITY UPLC~BEH C18column( 50 mm × 2. 1 mm,1. 7 µm) through a gradient elution of methanol-0. 04% aqueous phosphoric at a flow rate of 0. 3 m L/min. The detection wavelength was 242 nm, and the column temperature was set at30 ℃. Similarity evaluation system for chromatographic fingerprint of TCM( Version 2004 A) was used to analyze the fingerprints. Results: 13 common peaks were selected as the fingerprint peaks from 13 batches of Gentiana officinalis root samples, with the similarities between 0. 976 to 0. 997, and Gentiana macrophylla root used for fingerprint applicability test has high similarity( 0. 998) with Gentiana officinalis root. Conclusion: This method with good precision and reliability should be used for the quality control of Gentiana officinalis root.