Biochemical characterization of a stable azoreductase enzyme from Chromobacterium violaceum: Application in industrial effluent dye degradation.
Int J Biol Macromol
; 121: 1011-1018, 2019 Jan.
Article
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| MEDLINE
| ID: mdl-30342139
ABSTRACT
The presence of dye, including azo functional group (NN) containing dyes, in industrial waste water is one of the major causes of water pollution. This report showcases the functional role of azoreductase from Chromobacterium violaceum (MTCC No 2656) as a valuable enzyme for degradation of azo dyes. The enzyme was cloned, expressed, purified and biochemically characterized and further tested for degradation efficiency of azo group containing dyes like methyl red, amaranth and methyl orange. The degraded azo dye products (metabolites) resulted by the action of azoreductase enzyme had reduced toxicity on fibroblast cell lines (L929) as compared to raw and intact dye. Further, good stability of the enzyme makes it more suitable for various applications related to the degradation and decolourisation of effluent dyes.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Contaminantes Químicos del Agua
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Chromobacterium
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Colorantes
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Residuos Industriales
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NADH NADPH Oxidorreductasas
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2019
Tipo del documento:
Article
País de afiliación:
India