[Establishing Glioma-associated Oncogene Homolog 1 Genetic Lineage-tracing Mice for Studies on Hepatic Fibrosis].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 49(4): 560-565, 2018 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-30378310
ABSTRACT
OBJECTIVE:
To establish Gli1-CreERt2; tdTomato genetic lineage-tracing mice for studies on hepatic fibrosis.METHODS:
Offspring of ROSA26 td Tomato (tdTomato) mice and Gli1-CreERt2 mice (Gli1 mice) were obtained, with Gli1-CreERt2; tdTomato genotype being identified by PCR. The mice model of hepatic fibrosis was induced with CCl4. Their liver samples were taken. The formalin-fixed and paraffin-embedded samples were prepared for HE staining and Masson staining. The expression of tdTomato was observed under immunofluorescent microscope.RESULTS:
An ideal number of Gli1-CreERt2; tdTomato genetic lineage-tracing mice were harvested. The differences in fertility between the parental and the offspring mice were not significant (P>0.05). Pseudolobular formation occurred in the CCl4-induced hepatic fibrosis model mice. Enhanced red fluoresce was observed in the model mice.CONCLUSION:
Gli1-CreERt2; tdTomato genetic lineage-tracing mice can be used to monitor the cell source of fibrous tissues, its transition as well as the underlying mechanism of pathogenesis of hepatic fibrosis.Palabras clave
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Banco de datos:
MEDLINE
Asunto principal:
Proteína con Dedos de Zinc GLI1
/
Cirrosis Hepática
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2018
Tipo del documento:
Article
País de afiliación:
China