Quantitative CT and 19F-MRI tracking of perfluorinated encapsulated mesenchymal stem cells to assess graft immunorejection.
MAGMA
; 32(1): 147-156, 2019 Feb.
Article
en En
| MEDLINE
| ID: mdl-30535540
ABSTRACT
OBJECTIVES:
Peripheral artery disease (PAD) affects 12-14% of the world population, and many are not eligible for conventional treatment. For these patients, microencapsulated stem cells (SCs) offer a novel means to transplant mismatched therapeutic SCs to prevent graft immunorejection. Using c-arm CT and 19F-MRI for serial evaluation of dual X-ray/MR-visible SC microcapsules (XMRCaps) in a non-immunosuppressed rabbit PAD model, we explore quantitative evaluation of capsule integrity as a surrogate of transplanted cell fate. MATERIALS ANDMETHODS:
XMRCaps were produced by impregnating 12% perfluorooctylbromine (PFOB) with rabbit or human SCs (AlloSC and XenoSC, respectively). Volume and 19F concentration measurements of XMRCaps were assessed both in phantoms and in vivo, at days 1, 8 and 15 after intramuscular administration in rabbits (n = 10), by 3D segmenting the injection sites and referencing to standards with known concentrations.RESULTS:
XMRCap volumes and concentrations showed good agreement between CT and MRI both in vitro and in vivo in XenoSC rabbits. Injected capsules showed small variations over time and were similar between AlloSC and XenoSC rabbits. Histological staining revealed high cell viability and intact capsules 2 weeks after administration.CONCLUSIONS:
Quantitative and non-invasive tracking XMRCaps using CT and 19F-MRI may be useful to assess graft immunorejection after SC transplantation.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tomografía Computarizada por Rayos X
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Enfermedad Arterial Periférica
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Células Madre Mesenquimatosas
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Imagen por Resonancia Magnética con Fluor-19
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Flúor
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Rechazo de Injerto
Tipo de estudio:
Guideline
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
MAGMA
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos