Excision of selectable markers from the Escherichia coli genome without counterselection using an optimized &#955;Red recombineering procedure.

Bubnov, Dmitrii M; Yuzbashev, Tigran V; Vybornaya, Tatiana V; Netrusov, Alexander I; Sineoky, Sergey P

Excision of selectable markers from the Escherichia coli genome without counterselection using an optimized λRed recombineering procedure.

Bubnov, Dmitrii M; Yuzbashev, Tigran V; Vybornaya, Tatiana V; Netrusov, Alexander I; Sineoky, Sergey P.

Afiliación

Bubnov DM; Bioresource Center Russian National Collection of Industrial Microorganisms (BRC VKPM), State Research Institute of Genetics and Selection of Industrial Microorganisms of National Research Center "Kurchatov Institute", 1-st Dorozhny pr., 1, Moscow 117545, Russia; Department of Microbiology, Faculty
Yuzbashev TV; Bioresource Center Russian National Collection of Industrial Microorganisms (BRC VKPM), State Research Institute of Genetics and Selection of Industrial Microorganisms of National Research Center "Kurchatov Institute", 1-st Dorozhny pr., 1, Moscow 117545, Russia; Department of Bioengineering, Imperi
Vybornaya TV; Bioresource Center Russian National Collection of Industrial Microorganisms (BRC VKPM), State Research Institute of Genetics and Selection of Industrial Microorganisms of National Research Center "Kurchatov Institute", 1-st Dorozhny pr., 1, Moscow 117545, Russia.
Netrusov AI; Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, Lenin's Hills, 1-12, Moscow 119234, Russia.
Sineoky SP; Bioresource Center Russian National Collection of Industrial Microorganisms (BRC VKPM), State Research Institute of Genetics and Selection of Industrial Microorganisms of National Research Center "Kurchatov Institute", 1-st Dorozhny pr., 1, Moscow 117545, Russia. Electronic address: sineoky@genetika

J Microbiol Methods ; 158: 86-92, 2019 03.

Article en En | MEDLINE | ID: mdl-30738107

Asunto(s)

ADN; Escherichia coli/genética; Ingeniería Genética/métodos; Genoma Bacteriano; Recombinación Genética; Cromosomas Bacterianos; ADN Bacteriano/genética; Edición Génica; Mutación

Palabras clave

Counterselection; Recombineering; λRed-mediated genome engineering

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Recombinación Genética / ADN / Ingeniería Genética / Genoma Bacteriano / Escherichia coli Idioma: En Revista: J Microbiol Methods Año: 2019 Tipo del documento: Article

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