Calcium ionophore A23187 as a regulator of gene expression in mammalian cells.
J Cell Biol
; 103(6 Pt 1): 2145-52, 1986 Dec.
Article
en En
| MEDLINE
| ID: mdl-3097027
ABSTRACT
The calcium ionophore A23187 can reversibly induce the expression of two glucose-regulated genes, p3C5 and p4A3. This induction requires a continuous presence of the ionophore for over 2 h. Although extracellular Ca2+ is important for the optimal effect of A23187, it is not necessary for the induction, since a similar response with a lower magnitude can be triggered in cells cultured in low Ca2+ medium buffered with EGTA. Both the basal and induced levels of p3C5 and p4A3 transcripts can be modulated by the calmodulin antagonist W-7, indicating the involvement of Ca2+/calmodulin-associated pathways. In addition, the sensitivity of the A23187 induction to cycloheximide suggests that the induction process is dependent on de novo protein synthesis.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Genes Reguladores
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Calcimicina
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Proteínas HSP70 de Choque Térmico
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Genes
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Proteínas de la Membrana
Límite:
Animals
Idioma:
En
Revista:
J Cell Biol
Año:
1986
Tipo del documento:
Article