Exploring the impact of Mg-doped ZnO nanoparticles on a model soil microorganism Bacillus subtilis.
Ecotoxicol Environ Saf
; 182: 109421, 2019 Oct 30.
Article
en En
| MEDLINE
| ID: mdl-31301592
ABSTRACT
The environmental contamination of soil by metal oxide nanomaterials is a growing global concern because of their potential toxicity. We investigated the effects of Mg doped ZnO (Mg-nZnO) nanoparticles on a model soil microorganism Bacillus subtilis. Mg-nZnO exhibited only a moderate toxic effect on B. subtilis vegetative cells but was able to prevent biofilm formation and destroy already formed biofilms. Similarly, Mg-nZnO (≤1â¯mg/mL) was moderately toxic towards Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enterica, Saccharomyces cerevisiae and murine macrophages. Engineered Mg-nZnO produced H2O2 and O2â¢- radicals in solutions of various salt and organic molecule compositions. A quantitative proteomic analysis of B. subtilis membrane proteins showed that Mg-nZnO increased the expression of proteins involved in detoxification of ROS, translation and biofilm formation. Overall, our results suggest that Mg-nZnO released into the environment may hinder the spreading, colonization and biofilm formation by B. subtilis but also induce a mechanism of bacterial adaptation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Contaminantes del Suelo
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Bacillus subtilis
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Óxido de Zinc
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Nanopartículas
Límite:
Animals
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Año:
2019
Tipo del documento:
Article